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KMID : 0545120020120050773
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 5 p.773 ~ p.779
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Cloning and Expression of the Aminopeptidase Gene from the Bacillus licheniformis in Bacillus subtilis
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KIM, JIN SOOK
LEE, IN SOO/LEE, SEUNGWON/LEE, YOUNG-PHIL/JUNG, CHULHO/KIM, HYUNG CHEOL/CHOI, SOON-YONG
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Abstract
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A gene (bap) encoding aminopeptidase from the chromosomal DNA of Bacillus licheniformis was cloned. The gene is 1,347 bp long and encodes a 449 amino acid preproprotein with a major mature region of 401 amino acids (calculated molecular mass 43,241 Da). N-Terminal sequence of the purified protein revealed a potential presence of N-Terminal propeptide. The deduced primary amino acid sequence and the mass analysis of the purified protein suggested that a C-terminal peptide YSSVAQ was also cleaved off by a possible endogeneous protease. The amino acid sequence displayed 58% identity with that of the aminopeptidase from alkaliphilic Bacillus halodurans. This bacterical enzyme was overexpressed in recombinant Escherichia coli and Bacillus subtilis cells. Clones containing the intact bap gene, including its own promoter and signal sequence, gave rise to the synthesis of extracellular and thermostable enzyme by B. subtilis transformants. The secreated protein exhibited the same biochemical properties and the similar apparent molecular mass as the B. licheniformis original enzyme.
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